Restriction mapping in nanofluidic devices
نویسندگان
چکیده
منابع مشابه
Restriction mapping in nanofluidic devices.
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites wit...
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1 Institute of Nano Engineering and Microsystems, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan; E-Mail: [email protected] 2 Department of Engineering and System Science, National Tsing Hua University, Kuang Fu Road, No. 101, Section 2, Hsinchu 30013, Taiwan 3 Division of Mechanics, Research Center for Applied Sciences, Academia Sinica, Nankang, 11...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2005
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.0503809102